|
 |
Genomics Core
Genomics Core Facilities Available to the San Diego Community
The Genomics Core at Sidney Kimmel Cancer Center provides a service to the
local research community, which includes biotechnology companies, UCSD, TSRI,
The Burnham Institute, and SDSU. Faculty members on the Genomics/Bioinformatics
Core Committee are Dr. Michael McClelland, who studies bacteria and human
genomics and prostate cancer, and Dr. John Welsh, who works on genomics technology
development. The Core is equipped to process many types of samples using a BD BioMek
FX Liquid Handling Robot, Affymetrix GeneChip System, ABI 7900HT, GeneMachine
OmniGridder, and other equipment. The BD BioMek FX Liquid Handling Robot handles  samples
either in 96-well or 384-well microtiter plate format. Currently the
robot is used to set up more than 1,000 PCR reactions per hour with
minimum human intervention and for re-arraying samples for microarrays.
The Robot can also be used for advanced customized assays for high-throughput
experiments. The Affymetrix
GeneChip System is one of the most mature microarray
technologies current available to the research community. A variety
of GeneChips are available in the Affymetrix product lines, including
Human UniGene sets, Human SNPs, P450 SNPs, P53, mouse genome, mouse
neurobiology, mouse toxicology, and others. With its integrated data
analysis tools and databases, one can study over 10,000 genes from
one single experiment. The suite includes the Affymetrix Data Mining
Tool. The real-time PCR machine, ABI 7900HT, is capable of time-resolve
fluorescence and melting temperature detection. The machine is used
for quantitative RT-PCR for SNP detection. We also spot microarrays on microscope slides using a GeneMachine.
Up to 30,000 spots consisting of oligos or PCR amplified DNAs can be
spotted. Our resources include over 30,000 human EST PCR products,
25,000 rat ESTs, and 15,000 mouse ESTs, and the complete set of PCR
products for Salmonella typhimurium ORFs. The core is also supported by the Bioinformatics Core in data curation,
data analysis, databases, database applications. |
|
